Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia beta-1,3-glucanase gene.

Tissue-Specific and Pathogen-lnduced Regulation of a Nicotiana plumbaginifolia

The Nicotiana plumbaginifolia gnl gene encoding a 8-1,3-glucanase isoform has been characterired. The gnl product represents an isoform distinct from the previously identified tobacco 8-1,3-glucanases. By expressing gnl in Escherichia coli, we have determined directly that the encoded protein does, indeed, correspond to a &1,3glucanase. In N. plumbaginifolia, gnl was found to be expressed in ro...

Sequence of a Nicotiana plumbaginifolia beta(1,3)-glucanase gene encoding a vacuolar isoform.

/3(l,3)-glucanases are a group of hormonally and developmentaUy regulated hydrolytic plant enzymes, which are also induced upon pathogen infection. A cDNA and genomic clone of the Nicotiana plumbaginifolia /3(l,3)-glucanase gnl gene have been analyzed previously (1, 2). Here, we report the nucleotide and deduced amino acid sequences of a different N. plumbaginifolia 0(1,3)glucanase gene designa...

Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation.

Both cytokinin (N6-benzyladenine [BA]) and auxin (2,4-dichlorophenoxyacetic acid [2,4-D]) stimulate the accumulation of an mRNA, represented by the cDNA pLS216, in Nicotiana plumbaginifolia suspension culture cells. The kinetics of RNA accumulation were different for the two hormones; however, the response to both was transient, and the magnitude of the response was dose dependent. Runoff trans...

Understanding Tissue-Specific Gene Regulation.

Although all human tissues carry out common processes, tissues are distinguished by gene expression patterns, implying that distinct regulatory programs control tissue specificity. In this study, we investigate gene expression and regulation across 38 tissues profiled in the Genotype-Tissue Expression project. We find that network edges (transcription factor to target gene connections) have hig...

Sequence of the psbA gene from wild type and triazin-resistant Nicotiana plumbaginifolia.